Silvia Brunelli - Associate Professor

S. Brunelli 150x150 Department and institution: Department of Health Sciences, School of Medicine and Surgery, University of Milano Bicocca
tel. +39 02 64488308
Curriculum Vitae
institutional profile page: UNIMIB
see also: PubMed, BOA, ResearchGate, Scopus Author ID 7003321841

Keywords: Muscle regeneration, Tissue repair, Endothelial to Mesenchymal Transition, Endothelial Progenitors, Innate Immunity

Research interests

    Regeneration of muscle fibers, lost during pathological muscle degeneration or after injuries, is sustained by generation of new myofibers, Progenitors in the muscle comprise quiescent myogenic cells, the satellite cells, that are primary stem cells responsible for skeletal muscle regeneration after damage and vessel-associated progenitors, such as the mesoangioblasts (MAB). Using a genetic lineage tracing approach, a transgenic mouse expressing an inducible Cre under the control of an endothelial specific promoter we have provided new insights on the distinctive characteristics of the extraembryonic and embryonic hemogenic endothelium and we have identified for the first time the in vivo counterpart of embryonic MAB (Azzoni et al, 2014).
    We also shown that macrophages are necessary for efficient vascular remodeling in the injured muscle. When phagocyte infiltration is compromised endothelial-derived progenitors undergo a significant endothelial to mesenchymal transition (EndoMT). This together with an inefficient tissue remodeling contributes to the accumulation of collagen, fat and significant fibrosis. Our findings provide new insights in EndoMT in the adult skeletal muscle, and suggest that endothelial cells in the skeletal muscle may represent a new target for therapeutic intervention in fibrotic diseases (Zordan et al, 2014). We are extending these studies to another diseases, both of genetic and acquired origin such as Fibrodysplasia Ossificans Progressive (FOP) (Cappato et al, High throughput screening for modulators of ACVR1 transcription potentially applicable to the treatment of Fibrodysplasia Ossificans Progressiva, in revision Disease and Models and Mechanisms) and Systemic Sclerosis (Nicolosi et al, 2016).

Main topics of the group

    Molecular Mechanisms of Muscle regeneration, Endothelial to Mesenchymal Transition in Development and Diseases

Current research projects

    - Fondazione Cariplo Ricerca Medica 2013 Role of cripto in orchestrating tissue remodelling in muscle damage. ordinator of the project (2 units) and Unit PI
    - Italian Ministry of University PRIN 2012 Identificazione di nuove molecole terapeutiche per le malattie muscolari orfane su base infiammatoria. Unit PI
    - Telethon GGP15196 New treatment strategies for Fibrodysplasia Ossificans Progressiva PI

Internal and external collaborators

Selected pubblications
see also: PubMed, BOA

Selected Recent Publications

  • Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S., & Minchiotti, G. (2017).
    Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection.

  • Tirone, M., Tran, N., Ceriotti, C., Gorzanelli, A., Canepari, M., Bottinelli, R., et al. (2017).
    High mobility group box 1 orchestrates tissue regeneration via CXCR4.

  • Cappato, S., Tonachini, L., Giacopelli, F., Tirone, M., Galietta, L., Sormani, M., et al. (2016).
    High throughput screening for modulators of ACVR1 transcription potentially applicable to the treatment of Fibrodysplasia Ossificans Progressiva.

  • Nicolosi, P., Tombetti, E., Maugeri, N., Rovere-Querini, P., Brunelli, S., & Manfredi, A. (2016).
    Vascular Remodelling and Mesenchymal Transition in Systemic Sclerosis.

  • Tirone, M., Conti, V., Manenti, F., Nicolosi, P., D'Orlando, C., Azzoni, E., et al. (2016).
    Nitric Oxide Donor Molsidomine Positively Modulates Myogenic Differentiation of Embryonic Endothelial Progenitors.
    PLOS ONE, 11(10).


last update - May 2018

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