Chiara Lanzuolo - Researcher
Department and institution: Institute of Cell Biology and Neurobiology at Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi” INGM
tel. +39 02 00660358
see also: PubMed
personal web page: INGM - personal web page
Keywords: epigenetics, nuclear architecture, Polycomb, nuclear lamin, Emery Dreifuss Muscular Dystrophy, Progeria, aging, differentiation
Over the past 10 years a large number of mutations in the human lamin genes have been associated with numerous diseases collectively termed laminopathies. Although it is clear that structural organization and epigenetic regulation of chromatin are altered in diseases caused by mutant lamins, nothing is known about the specific functions of lamins in these processes under normal physiological conditions. The Polycomb group (PcG) of proteins is part of a conserved epigenetic cell memory system that prevents changes in cell lineage identity by maintaining transcription patterns through cell divisions via chromatin structure. We hypothesize an involvement of lamin in the coordination of gene expression regulating contemporary sub-set of genes throughout direct or indirect interactions with Polycomb epigenetic complexes. Our studies are focused on physiological processes such as differentiation and senescence and pathological conditions as premature senescence and lamin disorders to find the role Lamin A in the regulation of gene expression. To this aim we combine molecular and cellular biology, the use of high-resolution techniques, such as Chip, FISH, 4C and time-lapse microscopy supported by high performance computing for the analysis of data. The ultimate objective will be to produce the fundamental knowledge of functional interactions between Polycomb repressive complexes and Lamin A that could help to untangle the intricate cascade of events at the basis of lamin related diseases, providing new tools for preclinical studies.
Main topics of the group
In this laboratory, we study epigenetic mechanisms involved in lamin dependent disease with the following projects:
- Dissecting the role of Lamin A/C in genome organization and regulation
- Role of LaminA/C-Polycomb crosstalk in Emery Dreifuss Muscular Dystrophy
- Identification of epigenome alterations in Hutchinson-Gilford Progeria Syndrome
Current research projects
- Dissecting the role of Lamin A/C in genome organization and regulation (MIUR)
- Role of LaminA/C-Polycomb crosstalk in Emery Dreifuss Muscular Dystrophy (MIUR, MoH)
- Identification of epigenome alterations in Hutchinson-Gilford Progeria Syndrome (Epigen-CNR)
Internal and external collaborators
Dr Ferrari Francesco, IFOM-IEO, Milan, Italy
Dr Oliva Gennaro, Dr Antonelli Laura, Dr Gregoretti Francesco, ICAR-CNR, Naples, Italy
Dr Pasini Diego, IFOM-IEO, Milan, Italy
Dr Bearzi Claudia, MultiMedica, Milan, Italy
Dr Bodega Beatrice, INGM, Milan, Italy
Dr Bonne Giselle, Dr Muchir Antoine, Istitut de Myologie, Paris, France
Dr Schirmer Eric, Wellcome Trust Centre for Cell Biology, Edinburgh, UK
Dr Meinke Peter , Ludwig-Maximilians University, Monaco, Germany
- Marullo F., Cesarini E., Antonelli L., Gregoretti F., Oliva G. and Lanzuolo C
" Nucleoplasmic Lamin A/C and Polycomb group of proteins: an evolutionarily conserved interplay "
Nucleus. 2016 Mar 1: 1-9
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- Cesarini E, Mozzetta C, Marullo F, Gregoretti F, Gargiulo A, Columbaro M., Cortesi A, Antonelli L, Di Pelino S, Squarzoni S, Palacios D, Zippo A, Bodega B, Oliva G and Lanzuolo C
“Lamin A/C sustains PcG proteins architecture maintaining transcriptional repression at target genes”
J Cell Biol. 2015 Nov 9;211(3):533-51
- Bianchi A and Lanzuolo C
“Into the chromatin world: Role of nuclear architecture in epigenome regulation”
AIMS Biophysics 2015 Oct 27; 2(4): 585-612
- Lanzuolo C, Lo Sardo F, Diamantini A. Orlando V.
“PcG complexes set the stage for epigenetic inheritance of gene silencing in early S phase before replication” PLoS Genet. 2011 Nov;7(11):e1002370.
- Lanzuolo C, Roure V, Dekker J, Bantignies F, Orlando V.
“Polycomb response elements mediate the formation of chromosome higher-order structures in the bithorax complex” Nat Cell Biol. 2007 Oct 9(10):1167-74
last update: January 2017