Alessio Zippo - Researcher
Department and institution:
see also: PubMed
Keywords: Wnt/β-catenin Signalling; Epigenetics; Embryonic Stem Cells; Cancer Stem Cell; reprogramming; Myc oncogene.
We are currently focusing our attention on the molecular mechanisms through which the transcription factor Myc influences global chromatin structure in ESC using biochemical, molecular and genetic approaches. In particular we are determining the contribution of Myc in integrating environmental signals with the core regulatory transcription network.
On the same line, we are also investigating whether Myc plays a key role in determining the formation of Cancer Stem Cells (CSC) by favoring the establishment of a stem cell-like epigenetic signature. We are focusing on the dynamic epigenetic changes occurring at defined loci in primary mammary epithelial cells going through a Myc-induced reprogramming towards a primitive stem cell-like state.
In the same cellular context, we are also investigating the molecular mechanisms through which the activation of the Wnt/β-catenin pathway promotes the establishment and the maintenance of the mammary CSC. In particular, we will focus in analyzing the downstream effects of β-catenin activation at the chromatin level including the expression signature and the epigenetic state of the CSC.
Role of Myc in determining the epigenetic state of Embryonic Stem cell
Epigenetic mechanisms controlling the establishment of Cancer Stem Cells
Wnt/ β-catenin signaling in stem cells
see also: PubMed
A cytosolic Ezh1 isoform modulates a PRC2-Ezh1 epigenetic adaptive response in postmitotic cells.
Nat Struct Mol Biol. 2017 May;24(5):444-452. doi: 10.1038/nsmb.3392. Epub 2017 Mar 27.
Bodega B, Marasca F, Ranzani V, Cherubini A, Della Valle F, Neguembor MV, Wassef M, Zippo A, Lanzuolo C, Pagani M, Orlando V.
Multiple Roles of MYC in Integrating Regulatory Networks of Pluripotent Stem Cells.
Front Cell Dev Biol. 2017 Feb 3;5:7. doi: 10.3389/fcell.2017.00007. eCollection 2017.
Fagnocchi L, Zippo A.
Fluorescence Resonance Energy Transfer Microscopy for Measuring Chromatin Complex Structure and Dynamics.
Methods Mol Biol. 2016;1480:143-52. doi: 10.1007/978-1-4939-6380-5_13.
Cherubini A, Zippo A.
1. FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription. Mol Cell Biol. 2013 Mar;33(6):1198-209
Evellin S, Galvagni F, Zippo A, Neri F, Orlandini M, Incarnato D, Dettori D, Neubauer S, Kessler H, Wagner EF, Oliviero S.*
2. Myc regulates the transcription of PRC2 to control the expression of developmental genes in embryonic stem cells
Mol Cell Biol. 2012 Feb;32(4):840-51
Neri F, Zippo A, Krepelova A, Cherubini A, Rocchigiani M, and Oliviero S*
3. Histone crosstalk between H3S10ph and H4K16ac generates a histone code that mediates transcription elongation.
Cell. 2009 Sep 18;138(6):1122-36. doi: 10.1016/j.cell.2009.07.031.
Zippo A, Serafini R, Rocchigiani M, Pennacchini S, Krepelova A, Oliviero S.
4. PIM1-dependent phosphorylation of histone H3 at serine 10 is required for MYC-dependent transcriptional activation and oncogenic transformation.
Nat Cell Biol. 2007 Aug;9(8):932-44. Epub 2007 Jul 22.
Zippo A, De Robertis A, Serafini R, Oliviero S.
last update: January 2018